Download e-book for iPad: Alligator Metabolism Studies on Chemical Reactions in Vivo by Roland A. Coulson, Thomas Hernandez

By Roland A. Coulson, Thomas Hernandez

ISBN-10: 0080297765

ISBN-13: 9780080297767

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Extra resources for Alligator Metabolism Studies on Chemical Reactions in Vivo

Example text

It is perhaps too soon to tell which of the theories will receive final acceptance but most agree that an enzyme (Na-K-ATPase) and ATP are involved in electrolyte transport across the cell membrane. The cost of separating Na and Κ in the in vitro experi­ ments seemed to be very high when measured in iso­ lated cell systems metabolizing aerobically. Evidence for high cost was obtained as follows: individual cells respire and the amount of 0 2 used may be deter­ mined. This 0 2 is used for all of the usual life pro­ cesses, including furnishing energy for operation of what is called the Na-K-ATPase pump.

At the distal side of the cell the ratio of molecules of product to substrate re-entering the distal capillary will be constant in all animals, but the rate at which both re-enter the plasma will be proportional to the blood flow. An attempt has been made in Fig. 5 to illustrate what we believe to be the relationship between forward and backward reactions where E + S

1 mmol/g/min. 5 mmol/g/min. In other words, the dry weight of the glucose absorbed/min would approach half the dry weight of the transporting mucosa of the small intes­ tine. It would be difficult not to conclude that the ex­ pression for transport of a food metabolite across the gut mucosa or across other cell membranes should resemble that for enzyme reactions generally. If trans­ port involves a carrier molecule, the rate of formation of the complex, and the subsequent rate of its break­ down should be proportional to blood flow and to the substrate concentration.

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Alligator Metabolism Studies on Chemical Reactions in Vivo by Roland A. Coulson, Thomas Hernandez


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